RBS

Part:BBa_K2541210:Design

Designed by: Shuting Zheng   Group: iGEM18_Jilin_China   (2018-10-05)


Cold-repressible RNA-based thermosensor-10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The secondary structure is important to the function of an RNA thermosensor. We designed the thermosensor according to the stem length, loop size and base bulge.

Source

This sequence is not derived from any organism. We designed this sequence on our own and synthesized this sequence from a synthesis company.

References

[1]Pertzev A V, Nicholson A W. Characterization of RNA sequence determinants and antideterminants of processing reactivity for a minimal substrate of Escherichia coli ribonuclease III[J]. Nucleic Acids Research, 2006, 34(13):3708-3721.

[2]Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches.[J]. Nature Reviews Microbiology, 2012, 10(4):255-65.